Modifying Enzymes
LGC GENOMICS LLC (Lucigen) Exonuclease III, 25,000 U @ 200 U/LDigest duplex DNA in a 3 to 5 direction from a nick, blunt end, or 3-recessed end, producing stretches of ssDNA.
Produce stretches of ssDNA in dsDNA templates containing nicks, blunt ends or recessed 3' endsProtect protruding 3' ends because this exonuclease will not start digestion at protruding 3' overhangsThis product includes both Exonuclease III enzyme and a 10X reaction buffer.
Sigma Aldrich Fine Chemicals Biosciences Ribonuclease H RNase H fro
Ribonuclease H (RNase H) is a nonspecific endoribonuclease localized to the nucleus and cytoplasm. It is ubiquitously found and widely present among many organisms including viruses and human.
New England Biolabs, Inc. PvuII-HF – 5000 units
A high fidelity restriction endonuclease that recognizes the sequence CAG^CTG. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.
New England Biolabs, Inc. Endoproteinase AspN – 50 µg
Endoproteinase AspN (flavastacin) is a zinc metalloendopeptidase which selectively cleaves protein and peptide bonds N-terminal to aspartic acid residues.
STEMCELL Technologies Elastase, Size: 25 mg
Elastase is a serine protease consisting of a single polypeptide chain of 240 amino acid residues and contains four disulfide bridges (Shotton & Hartley). Elastase is synthesized as a zymogen (proelastase) and is converted to the active form by limited trypsin proteolysis at its N-terminal. Elastase preferentially cleaves the peptide bond of C-terminal neutral, non-aromatic amino acid residues (Schellenberger et al.). Elastase is frequently used in combination with other proteases such as collagenase and trypsin to digest fibrous tissues because of its ability to digest native elastin.
XenoTech,LLC Mouse CYP3A11 Low Reductase Bactosomes, 1nmol/vial
Mouse CYP3A11 Low Reductase Bactosomes, 1nmol/vial
XenoTech,LLC Mouse CYP2B10 Low Reductase Bactosomes, 1nmol/vial
Mouse CYP2B10 Low Reductase Bactosomes, 1nmol/vial
XenoTech,LLC EasyCYP CYP4F3B Low Reductase 0.5 nmol P450 at 1 nmol/ml, 10 mg/ml protein
EasyCYP CYP4F3B Low Reductase 0.5 nmol P450 at 1 nmol/ml, 10 mg/ml protein
XenoTech,LLC CYP2C9 3R+ 1 NMOL
CYP2C9*3 Reductase Bactosomes, plus b5, Human rCYP Enzyme, 1 nmol/vial.
XenoTech,LLC CYP3A7 Reductase Bactosomes, Human rCYP Enzyme, 1 nmol/vial
CYP3A7 Reductase Bactosomes, Human rCYP Enzyme, 1 nmol/vial
XenoTech,LLC CYP2C8LR+ 1 NMOL
CYP2C8 Low Reductase Bactosomes, plus b5, Human rCYP Enzyme, 1 nmol/vial.